The embodiment of the invention provides a medical sensor and a manufacturing method thereof. The medical sensor comprises a substrate, detection electrodes, electrode wires and artificial bristles, wherein the detection electrodes are arranged on the substrate and are used for detecting bioelectricity of the human body, the electrode wires are arranged on the substrate and are used for electrically connecting the detection electrodes to signal collecting equipment or signal processing equipment, the artificial bristles are arranged on the substrate and are densely distributed around the detection electrodes, and each artificial bristle is adsorbed on the skin surface of the human body through molecular attraction. According to the scheme, as adsorption of the medical sensor is achieved by means of molecular attraction of the artificial bristles, the medical sensor can be used many times, and adsorption will not be affected by utilization for many times; a great number of gaps exist when the artificial bristles make contact with the skin surface of the human body, air permeability is high, when used for detecting the bioelectricity of the human body, the medical sensor does not affect breath of the skin, and the comfort degree of a testee is high.