The invention provides a method and a kit for detecting VHL gene mutation, and relates to the field of gene detection. The method comprises the steps: amplification primers of three exons of a VHL gene is utilized to detect VHL gene point mutation, small fragment deletion or insertion, and splice site mutation of a sample to be detected, thus obtaining detection results of whether point mutation, small fragment deletion or insertion, and splice site mutation exist in a DNA solution; a hybridization probe is used for detecting VHL gene large fragment deletion of the sample to be detected, thus obtaining a detection result of whether large fragment deletion exists in the sample to be detected. The invention also provides a kit containing the hybridization probe. According to the method, the amplification primers which are high in specificity and sensitivity and the hybridization probe are adopted for quickly and accurately detecting VHL gene mutation, the detection efficiency is high, the method is simple, and the result is accurate; the kit is low in cost, high in detection efficiency and good in detection effect.