The present invention discloses an application method of betulinic acid as a poultry feed additive. The betulinic acid is used as a feed additive to be added into laying hen feed with an addition amount of 10-100 mg/kg. The betulinic acid can increase the egg production rate, affect lipid metabolism, and improve egg quality. The betulinic acid as the poultry feed additive is less in addition amount, is safe, is obvious in addition effect, has no drug resistance, and is low in price.