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CN104946673A 对Bt毒素具有杀虫增效作用的小菜蛾碱性磷酸酶基因蛋白片段PxALP1及应用 失效 - 权利终止

对Bt毒素具有杀虫增效作用的小菜蛾碱性磷酸酶基因蛋白片段PxALP1及应用
摘要：
一种对Bt毒素具有杀虫增效作用的小菜蛾碱性磷酸酶基因蛋白片段PxALP1及应用，该蛋白片段序列如SEQ ID No.2所示，编码该蛋白的基因PxALP0的核苷酸序列如SEQ ID No.1所示，该基因PxALP0是从小菜蛾(Plutella xylostella)中克隆的一种新型的增效因子碱性磷酸酶片段，通过原核表达与毒力测定，表明该碱性磷酸酶片段PxALP0编码的多肽片段PxALP1具有显著提高Cry1Ac杀虫晶体蛋白对小菜蛾的杀虫活性，并可克服或延缓小菜蛾等鳞翅目害虫对Bt制剂或Bt转基因植物的抗性，在鳞翅目害虫的防治方面具有极为广泛的应用价值。
摘要（英）：

The invention discloses a plutella xylostella alkaline phosphatase gene protein fragment PxALP1 having an insecticidal enhancing effect on Bt toxins and applications thereof. The sequence of the protein fragment is shown in SEQ ID No.2, the nucleotide sequence of a gene PxALP0 encoding the protein is shown in SEQ ID No.1, and the gene PxALP0 is a novel enhancing factor alkaline phosphatase fragment cloned in plutella xylostella. Prokaryotic expression and toxicity tests show that a polypeptide fragment PxALP1 encoded by the alkaline phosphatase fragment PxALP0 significantly improves the insecticidal activity of Cry1Ac insecticidal crystal proteins to plutella xylostella, and can overcome or delay the resistance of lepidoptera pests such as plutella xylostella to Bt preparations or Bt transgenic plants, therefore, the protein fragment PxALP1 has extremely extensive application values in the prevention and control of lepidoptera pests.

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