The invention relates to a method for batch preparation of graphene from microcrystalline graphite, and belongs to the technical field of graphene material preparation. The method comprises the following steps: uniformly mixing microcrystalline graphite powder with a surfactant and a solvent 1 to the concentration of the microcrystalline graphite of 0.1-5mg.mL<-1>; carrying out ultrasonic treatment under an ultrasonic power of 200-3200W for 5-20h; carrying out crushing peeling in a vortex crusher with the power of 4000-6000W for 5-12h; carrying out ultrasonic treatment under the ultrasonic power of 200-3200W at 0-5DEG C for 5-20h; centrifuging, and collecting the obtained precipitate; dispersing the precipitate in a solvent 2, centrifuging, and collecting the new precipitate; dispersing the new precipitate, centrifuging, and collecting the finally obtained precipitate; and dispersing the finally obtained precipitate in water, freezing within 10min to form a solid, and carrying out vacuum drying to obtain microcrystalline graphene. The method has the advantages of simplicity, reliability, low cost and abundant reserve of the raw material microcrystalline graphite, and suitableness for batch production.