The invention discloses a half antigen SPE-EDE and a corresponding artificial antigen and application of the half antigen. A protection formula (I) shows a compound. A method that a compound is shown in a preparation formula (I) is provided, and the method comprises the following steps: spectinomycin hydrochloride is made to react with ethylene glycol bis(2,3-epoxypropyl) ether, and the compound shown in the formula (I) is generated; the compound shown in the formula (I) and conjugate (the artificial antigen) of carrier protein are further provided. The antibody obtained by serving the any conjugate as the immunogen is provided. The invention further provides a kit. The kit comprises the conjugate and the antibody. The invention further provides any conjugate or the antibody or the application of the knit to detection of spectinomycin or spectinomycin derivatives (like spectinomycin hydrochloride). The application has the great practical value in detection of the spectinomycin and the spectinomycin derivatives, operation is easy, the cost is low, and the requirement of a detection limit can be met. The formula (I) is shown in the description.