The invention relates to a surface molecular imprinting polymer employing a nanoflower material as a matrix, and the preparation and application of the surface molecular imprinting polymer. In a hole-forming solvent, under the action of a cross-linking agent, vinyl pyridine, methacrylic acid or acrylamide serves as a functional monomer, and the surface molecular imprinting polymer employing a nanoflower porous material as the matrix is obtained by grafting reaction on the outer surface of the nanoflower material and the surface of an internal hole. The surface molecular imprinting polymer made from the nanoflower material is highly capable of selectively adsorbing template molecules and has high stability and excellent dynamic adsorption performance.