The invention discloses a three-dimensional box loading method based on a three-dimensional moving mode sequence and a memetic algorithm. The method mainly solves the problem of low utilization rate on the volume of a three-dimensional box loading container in the prior art. The three-dimensional box loading method comprises the following realization steps that 1, each parameter is set; 2, an initial population is randomly generated, and the adaptive fitness of individuals in the population is calculated; 3, whether the termination conditions are met or not is judged, if so, the step 4 is executed, and otherwise, the step 9 is executed; 4, a binary tournament method is used for selecting the individuals; 5, the individuals are crossed, and the individual adaptive fitness value is calculated again; 6, the individuals are subjected to variation, and the individual adaptive fitness value is calculated again; 7, the individual with the greatest adaptive fitness value in the current generation is stored; 8, the number of the iteration times is added to 1, and the operation returns to the step 3; 9, a hill climbing method is used for optimizing the individuals with the greatest adaptive fitness value, and the optimized box loading result is output. The method has the advantages that the volume utilization rate of the container can be improved, and the method can be used for solving the box loading problem, and can also be used for soling other combination optimization problems.