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发明公开
CN104498491A 一种嗜热链球菌的特异性分子标记DNA序列及其用途 有权

一种嗜热链球菌的特异性分子标记DNA序列及其用途
摘要：
本发明公开了一种嗜热链球菌(Streptococcus thermophilus)的特异性分子标记DNA序列及其用途以及嗜热链球菌的检测方法。该特异性分子标记DNA序列如SEQ ID NO.6所示。该检测方法包括步骤：(1)提取待测样本的基因组DNA；(2)以步骤(1)所得的基因组DNA为模板，以SEQ ID NO.1所示的半随机引物为扩增引物进行单引物PCR扩增；(3)对步骤(2)所得扩增产物进行克隆测序，并将测序结果与SEQ ID NO.6所示序列进行比对，若同源度大于99％，则表明待测样本中含有嗜热链球菌。
摘要（英）：

The invention discloses a DNA sequence of a specific molecular marker of streptococcus thermophilus and use thereof as well as a detection method of streptococcus thermophilus. The DNA sequence of the specific molecular marker is shown in SEQ ID NO. 6. The detection method comprises the following steps: (1) extracting the genome DNA of a to-be-detected sample; (2) carrying out single primer PCR amplification by taking the genome DNA obtained in the step (1) as a template and a semi-random primer shown in the SEQ ID NO. 1 as an amplification primer; (3) clone-sequencing an amplification product obtained the step (2) and comparing the sequencing result with the sequence shown in the SEQ ID NO. 6; if the homologous degree is greater than 99%, showing that the to-be-detected sample contains streptococcus thermophilus.

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