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发明公开
CN104480136A 构建产纤维素酶和纳他霉素的重组利迪链霉菌的方法 无效 - 驳回

构建产纤维素酶和纳他霉素的重组利迪链霉菌的方法
摘要：
本发明公开了构建产纤维素酶和纳他霉素的重组利迪链霉菌的方法。本发明所提供的构建产纤维素酶和纳他霉素的重组利迪链霉菌的方法中，构建产纤维素酶和纳他霉素的重组利迪链霉菌的方法，包括将血红蛋白的编码基因和纤维素酶的编码基因导入作为宿主菌的利迪链霉菌中筛选得到产纤维素酶和纳他霉素的重组利迪链霉菌的步骤；血红蛋白为A1)氨基酸序列是序列表中SEQ ID No.3的蛋白质，或A2)具有血红蛋白功能的由A1)衍生的蛋白质；纤维素酶为B1)氨基酸序列是序列表中SEQ ID No.4的蛋白质，或B2)具有纤维素酶功能的由B1)衍生的蛋白质。
摘要（英）：

The invention discloses a construction method of recombinant streptomyces lydicus for producing cellulase and natamycin. The construction method of the recombinant streptomyces lydicus for producing cellulase and natamycin comprises the step as follows: an encoding gene of hemoglobin and an encoding gene of cellulase are guided into streptomyces lydicus as a host strain and screened to obtain the recombinant streptomyces lydicus for producing cellulase and natamycin, wherein hemoglobin is protein of A 1) or A 2), in A 1), the amino acid sequence of the protein is represented as SEQ ID No.3 in a sequence table, and in A 2), hemoglobin is protein which has the hemoglobin function and is derived from A 1); and cellulase is protein of B 1) or B 2), in B 1), the amino acid sequence of the protein is represented as SEQ ID No.4 in the sequence table, and in B 2), cellulase is protein which has the cellulase function and is derived from B 1).

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