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发明公开
CN104480135A 产纤维素酶和产纳他霉素的重组利迪链霉菌与应用 无效 - 驳回

产纤维素酶和产纳他霉素的重组利迪链霉菌与应用
摘要：
本发明公开了产纤维素酶和产纳他霉素的重组利迪链霉菌与应用。本发明所提供的重组利迪链霉菌的构建方法，包括将血红蛋白的编码基因和纤维素酶的编码基因的表达载体导入利迪链霉菌中，得到产纤维素酶和纳他霉素的重组利迪链霉菌的步骤；血红蛋白为A1)氨基酸序列是序列表中SEQ ID No.3的蛋白质，或A2)具有血红蛋白功能的由A1)衍生的蛋白质；纤维素酶为B1)氨基酸序列是序列表中SEQ ID No.4的蛋白质，或B2)具有纤维素酶功能的由B1)衍生的蛋白质；所述表达载体从上游至下游的方向含有红霉素抗性基因启动子、所述纤维素酶的编码基因、所述红霉素抗性基因启动子、所述血红蛋白的编码基因。
摘要（英）：

The invention discloses recombinant streptomyces lydicus for producing cellulase and natamycin and an application. A construction method of recombinant streptomyces lydicus comprises steps as follows: expression vectors of an encoding gene of hemoglobin and an encoding gene of cellulase are guided into streptomyces lydicus to obtain the recombinant streptomyces lydicus for producing cellulase and natamycin; hemoglobin is protein of A 1) or A 2), in A 1), the amino acid sequence of hemoglobin is represented as SEQ ID No.3 in a sequence table, and in A 2), hemoglobin is protein which has the hemoglobin function and is derived from A 1); and cellulase is protein of B 1) or B 2), in B 1), the amino acid sequence of cellulase is represented as SEQ ID No.4 in the sequence table, and in B 2), cellulase is protein which has the cellulase function and is derived from B 1). The expression vectors comprises an erythromycin resistance gene promoter, the encoding gene of cellulase, an erythromycin resistance gene promoter and the encoding gene of hemoglobin in the direction from upstream to downstream.

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