The invention discloses a method for rapidly preparing of Feeder cells by using a stirring-type cell culturing bottle rolling machine. The method comprises the three steps of the primary acquisition of CF-1 MEFs, the subculturing of CF-1 MEFs and the preparation of a CF-1 MEF Feeder. During the preparation of the CF-1 MEF Feeder, the stirring-type cell culturing bottle rolling machine serves as cell processing equipment, and a stirring hammer of the stirring-type cell culturing bottle rolling machine rotates constantly under the action of an electromagnetic field, so that cells can fully contact MMC in a culturing solution in a suspension state, and thus the efficiency of inhibiting the proliferation of the cells by using the MMC can be improved, and the cell processing time can be shortened. The special culturing bottle of the stirring-type cell culturing bottle rolling machine is several or hundreds of times the volume of a common culturing bottle/utensil and can be used repeatedly, and thus a large number of culturing bottles/utensils can be saved, the preparation cost of the Feeder can be reduced, the preparation efficiency of the Feeder can be improved, and a lot of time spent in doing experiments can be saved. In addition, according to the method, the cells are processed in a suspension state, and the cells do not need to be treated by using a digestive solution when being recovered, and thus the damage to the cells caused by digestion can be alleviated, and the cells can be kept in good states to the maximum extent.