The invention discloses a device for preparing and measuring a frozen soil sample. The uniform frozen soil sample can be prepared in a room, the thermal parameters and the permeability parameters of the frozen soil sample can be measured under the in-situ interference-free conditions, and a scientific basis and a technical support are provided for the accumulation evaluation of hydrates in a frozen soil area and the formulation of a hydrate exploitation scheme. The device comprises a frozen soil sample preparing unit, a permeability parameter measuring unit and a thermal parameter measuring unit; the frozen soil sample preparing unit comprises a pressure chamber, a confining pressure loading cavity, a rubber tube, a rotating table and a temperature control room; the permeability parameter measuring unit comprises a gas flow meter, a gas inlet, a gas outlet and a pressure sensor; the thermal parameter measuring unit comprises a heater, a temperature controller, a temperature sensor and a temperature pressure sensor. The invention also provides a method adopting the device.