The invention discloses a refining method of low-content 2-methylimidazole and belongs to the technical field of chemical synthesis. The refining method comprises the following steps: putting low-concentration 2-methylimidazole obtained in the synthesis reaction of 2-methylimidazole and water in a weight ratio of (1.2-1.8) to 1 into a reaction vessel, wherein the mass concentration of low-concentration 2-methylimidazole is 50-88%; stirring, heating to 80-85 DEG C, carrying out heat preservation for 30-40 minutes until the solid substance is dissolved, adding activated charcoal, continuously stirring for 15-25 minutes and filtering to obtain a filtrate; adding the filtrate into a preheated crystallization tank, carrying out heat preservation and standing for 20-30 minutes until the filtrate is layered, firstly separating a lower-layer base layer and then separating an intermediate-layer black flocculent substance and reserving the upper layer, stirring, and cooling and crystallizing to obtain high-content 2-methylimidazole. The refining method disclosed by the invention has the advantages of simple process, low cost, environmental friendliness, high purity and the like.