The invention discloses preparation and application of a test strip for detecting the clusterin content of urine, and belongs to the technical field of disease detection. The test strip is used for monitoring acute kidney injury and the renal toxicity safety of drugs. According to the clusterin colloidal gold test strip provided by the invention, the basic principle is a double-antibody sandwich method, a gold mark pad and an absorption pad are arranged at the two ends of a nitrocellulose membrane respectively, a sample pad is arranged at the upper end of the gold mark pad, the gold mark pad is coated with the colloidal gold coupling marker of a purified monoclonal clusterin antibody 1, a detection line is coated with a purified monoclonal clusterin antibody 2, a quality control line is coated with a normal goat anti-mouse IgG (immunoglobulin G) antibody, and the absorption pad is attached to the side of the quality control line. The test strip is easy to operate, an instrument is not required, a result is easy to judge, and a non-invasive detection method is adopted; the monoclonal antibodies for preparing the test strip are self-prepared by a hybridoma technology, so that the preparation is low in expense and suitable for industrialized production.