The invention relates to a rice iron-deficiency induction promoter and application thereof. The rice iron-deficiency induction promoter has a nucleotide sequence shown in i) SEQ ID NO.1, or ii) nucleotide sequence which is derived from the nucleotide sequence shown in SEQ ID NO.1 through substituting, deleting and/or adding one or more nucleotides and has an equivalent function. The invention further provides a carrier containing the promoter, a transgenic cell line and an engineering bacterium. The invention further provides application of the promoter to promoting the expression of a target gene. The invention further provides application of the promoter to a transgenic plant. A method of applying the promoter to the transgenic plant comprises the step of introducing a recombinant carrier into the target plant, thereby promoting the expression of a GUS gene by using a DNA segment in the target plant. The rice iron-deficiency induction promoter has the beneficial effect that the discovery of the promoter and the explanation of a function of the promoter have an important theory and practice significance for researching a rice tolerance iron-deficiency stress physiological mechanism and effectively developing rice iron-deficiency environment stress resistance and iron-rich breeding.