A cryopreservation method for a two-dimension nano-powder dispersion solution comprises the steps: step 1, adding two-dimension nano-material sheets into a mixed solution to prepare a dispersion solution with a predetermined concentration, and mixing for a period; step 2, performing centrifugation processing on the above dispersion solution, after the processing is finished, standing for a period, and then transferring the supernatant to a container; step 3, employing a low-temperature liquid gas to rapidly cool the two-dimension nano-powder to obtain a refrigerated powder; step 4, transferring the refrigerated powder of the dispersion solution into a low-temperature apparatus for storage, and controlling the storage temperature to be lower than the freezing point of the dispersion medium; and step 5, transferring the refrigerated powder out from the low-temperature apparatus, and unfreezing to form the two-dimension nano-powder dispersion solution again. The two-dimension nano-powder dispersion solution forms a solid for long-term storage by employing a low-temperature liquid gas for rapid refrigeration, so that the method helps to effectively avoid the easily-appeared agglomeration and re-stacking problems of the two-dimension nano-powder dispersion solution in a liquid medium, and is beneficial for device manufacturing, standardization and micromation of nano-materials.