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发明公开
CN103936853A 一种检测TIM-3试剂盒及其使用方法 有权

一种检测TIM-3试剂盒及其使用方法
摘要：
本发明属于生物工程技术领域，公开了一种检测TIM-3试剂盒及使用方法，本发明还公开用于检测Tim-3的单克隆抗体或其片段，所述抗体或其片段包括轻链CDR1、轻链CDR2、轻链CDR3、重链CDR1、重链CDR2和重链CDR3，各个CDR具有SEQ ID NO：1、SEQ ID NO：2、SEQ ID NO：3、SEQ ID NO：4、SEQ ID NO：5或SEQ ID NO：6所示氨基酸序列。本发明提供的单克隆抗体能够特异性与人Tim-3相结合，用所述抗体制备的检测人Tim-3的试剂盒，具有较高的灵敏度，能够检测出较低浓度的人Tim-3。
摘要（英）：

The invention belongs to the technical field of biotechnology, and discloses a kit used for detecting Tim-3, and an application method thereof. The invention also discloses monoclonal antibodies used for detecting Tim-3, or fragments of the monoclonal antibodies; the monoclonal antibodies or the fragments comprise light chain CDR1, light chain CDR2, light chain CDR3, heavy chain CDR1, heavy chain CDR2, and heavy chain CDR3; amino acid sequences of light chain CDR1, light chain CDR2, light chain CDR3, heavy chain CDR1, heavy chain CDR2, and heavy chain CDR3 are represented by SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, or SEQ ID No.6 respectively. The monoclonal antibodies are capable of realizing specific binding with human Tim-3; the kit which is prepared from the monoclonal antibodies and is used for detecting human Tim-3 possesses relatively high sensitivity, and can be used for detecting of human Tim-3 with low concentration.

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