The invention provides a method for cultivating Tibetan kefir strains. The method is characterized by including the following steps that first, a culture medium is prepared, wherein 50 parts to 100 parts of saw dust, 30 parts to 40 parts of manioc waste, 10 parts to 20 parts of grains and 5 parts to 12 parts of white granulated sugar are taken and evenly mixed, the mixture is boiled and disinfected for 40 minutes to 60 minutes at the high temperature of 100 DEG C to 120 DEG C and the high pressure of 30 MPa to 40 MPa, and then the mixture is naturally cooled to room temperature at normal temperature; second, Tibetan kefir is cultivated; third, domestication is performed. The Tibetan kefir strains cultivated with the method are used for making yogurt, no thickening agent or sweetening agent or additive is used, and the method is simple and high in operability and has wide market prospects.