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CN103760353B 检测稻曲菌素A的方法及其专用酶联免疫试剂盒 失效 - 权利终止 转让

检测稻曲菌素A的方法及其专用酶联免疫试剂盒
摘要：
本发明公开了一种检测稻曲菌素A的方法及其专用酶联免疫试剂盒。本发明所提供的方法及其专用试剂盒所使用的抗稻曲菌素A的单克隆抗体是由小鼠杂交瘤细胞株2D3G5分泌产生的，该小鼠杂交瘤细胞株在中国微生物菌种保藏管理委员会普通微生物中心的登记入册编号为CGMCC No.8774。本发明所提供的抗稻曲菌素A的单克隆抗体是以稻曲菌素A-OVA（稻曲菌素A和卵清白蛋白的偶联物）为包被抗原，通过间接竞争ELISA方法对杂交瘤细胞进行筛选得到的；利用该单克隆抗体制备得到的间接竞争酶联免疫试剂盒，用于定性或定量检测植物样品中的稻曲菌素A，线性检测范围在2.8-72.0ng/mL，具有快速、特异性强、灵敏度高的优点。
摘要（英）：

The invention discloses a method and a special ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting ustiloxin A. An ustiloxin A monoclonal antibody for the method and the special kit is secreted by a mouse hybridoma cell strain 2D3G5 with a registration number of CGMCC No. 8774 in the China general microbiological culture collection center, and is obtained by screening hybridoma cells by using an indirect competition ELISA method, and ustiloxin A-OVA (a conjugate of ustiloxin A and ovalbumin) serves as a coating antigen of the ustiloxin A monoclonal antibody; an indirect competition ELISA kit prepared by the monoclonal antibody is used for qualitatively or quantitatively detecting the ustiloxin A in a plant sample, and has the advantages of high speed, specificity and sensitivity, and the linear detection range is 2.8 to 72.0ng/mL.

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