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发明授权
CN103589698B 郁金香查尔酮合成酶TfCHS蛋白及其编码基因 失效 - 权利终止

郁金香查尔酮合成酶TfCHS蛋白及其编码基因
摘要：
本发明涉及一种郁金香查尔酮合成酶TfCHS蛋白及其编码基因，所述蛋白质为如SEQ ID NO.4所示的氨基酸序列组成的蛋白质或SEQ ID NO.4所示的氨基酸序列经过取代、缺失或者添加一个或几个氨基酸且具有郁金香查尔酮合成酶活性的蛋白质。本发明还提供了一种编码上述蛋白质的如SEQ ID NO.3所示的核酸序列。将本发明中郁金香查尔酮合成酶TfCHS基因通过构建正反义表达载体转化到烟草中后，转基因烟草查尔酮合成酶基因表达均受到不同程度的抑制，与此同时，转基因烟草花朵的颜色出现不同程度的变浅，对应的花朵总花色苷含量下降了20%‑60%。
摘要（英）：

The invention relates to a tulip chalcone synthase TfCHS protein, and a coding gene and a probe thereof. The protein is (a) or (b) as follows: (a) a protein composed of an amino acid sequence shown as a SEQ ID NO.4; and (b) a protein obtained by substitution, deletion or addition of one or several amino acids on the amino acid sequence shown as the SEQ ID NO.4, having tulip chalcone synthase activity and derived from (a). The invention also provides a nucleic acid sequence encoding the protein, and the probe to detect the above nucleic acid sequence. The invention provides theoretical basis for regulation of spatio-temporal expression characteristic of the TfCHS gene of tulip for color change and color innovation by using a genetic engineering technology, and has important application value.

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