The invention discloses a preparation method of a flexible displayer and a substrate used for manufacturing the flexible displayer. The method comprises the following steps: a layer of inorganic layer is deposited on the surface of carrier glass to act as a sacrificial layer; channels which are used for fixing an organic polymer thin film in an auxiliary way are etched on the sacrificial layer, and the sacrificial layer is cleaned; the channels are enclosed on the surface of the sacrificial layer so that a processing area used for processing a flexible displayer device is formed; one surface, which is provided with the channels, of the sacrificial layer is coated with organic polymer medicinal liquid so that an organic polymer thin film layer is formed; a TFT device and a light-emitting device are processed on the organic polymer thin film layer; packaging is performed; and the organic polymer thin film layer and the sacrificial layer are separated via a cutting mode so that the flexible displayer is formed. With application of the method, the flexible substrate is not liable to fall off from a base in the process of preparation, and the flexible substrate is easy to strip off from the base after completion of preparation so that the method is easy and convenient to operate and low in cost, and has no harmful effect on the light-emitting device.