The invention relates to a high expression production process and an extraction method for piglet escherichia coli K88, K99 and 987P cilium antigens. The production process is characterized in that glucose and peptone are fed in the culture process under a certain condition of temperature, stirring speed, ventilation volume and pH value, so as to obtain the escherichia coli with high expression cilium antigens, wherein the peptone is preferably tryptone. According to the production process, the escherichia coli with high expression K88, K99 and 987P cilium antigens can be obtained through dynamically feeding the glucose and the peptone under a certain condition of temperature, stirring speed, ventilation volume and pH value, the culture medium is low in cost, the fermentation process is simple and easy, and the solid foundation is laid for industrial production of piglet yellow scour vaccines.