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发明授权
CN103320525B HBV表型耐药检测试剂盒及其制备方法 有权 转让

HBV表型耐药检测试剂盒及其制备方法
摘要：
本发明公开了一种HBV表型耐药检测试剂盒，该试剂盒包括可以置换HBV1.0片段的重组入门载体pcDNA6.2‑HBV1.1‑sapI载体，其碱基序列如SEQ ID No.4所示，和带有分泌性碱性磷酸酶的双表达载体pcDNA5AP‑attR载体，其碱基序列如SEQ ID No.6所示。本发明提供的耐药检测试剂盒主要包括一套基于GatewayTM重组的表达载体系统，该重组系统避免了限制性内切酶位点对克隆地限制，可以快速、正确地将目的片段引入表达载体，并且可利用培养基上清进行化学发光定量检测，不仅灵敏度高而且可以根据标准碱性磷酸酶的对照进行定量和校准，操作简便，稳定。
摘要（英）：

The invention discloses a HBV phenotype drug resistance detection kit, which comprises a recombinant entry vector pcDNA6.2-HBV1.1-sapI capable of replacing a HBV1.0 fragment and a double expression vector pcDNA5AP-attR having secreted alkaline phosphatase, wherein a base sequence of the recombinant entry vector pcDNA6.2-HBV1.1-sapI is represented by SEQ ID No.4, and a base sequence of the double expression vector pcDNA5AP-attR is represented by SEQ ID No.6. The drug resistance detection kit mainly comprises a set of an expression vector system based on GatewayTM recombination, wherein the recombination system has the following characteristics that: limitation of restriction endonuclease sites on clone can be avoided, a target fragment can be rapidly and correctly introduced into an expression vector, and a culture medium supernatant can be adopted to carry out chemiluminescence quantitative detection, such that sensitivity is high, quantification and calibration can be performed according to the standard alkaline phosphatase control, and operation is convenient and stable.

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