The invention discloses an alpinia kwangsiensis compound, as well as a preparation method and application thereof. The invention provides a chemical structural formula of the compound separated from alpinia kwangsiensis, and the compound is prepared from the root part of the alpinia kwangsiensis, has nematicidal activity and can be widely applied to preparing nematicidal agents. The preparation method disclosed by the invention has the advantages of simplicity, low preparation cost and broad application prospects.