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发明公开
CN103097538A 支链脂肪酸以及其生物性生产 失效 - 权利终止

支链脂肪酸以及其生物性生产
摘要：
本发明提供了生产反异脂肪酸的方法。所述方法包括在允许一种或更多种多核苷酸的表达和反异脂肪酸的生产的条件下培养细胞，所述细胞包含至少一种外来的或过表达的多核苷酸，所述多核苷酸包含编码多肽的核酸序列，所述多肽催化下列反应中的至少一种：丙酮酸至柠苹酸的转化；柠苹酸至柠康酸的转化；柠康酸至β-甲基-D-苹果酸的转化；β-甲基-D-苹果酸至2-氧代丁酸的转化；或苏氨酸至2-氧代丁酸的转化。任选地，所述细胞还包含至少一种外来的或过表达的多核苷酸，所述多核苷酸包含编码多肽的核酸序列：所述多肽催化下列反应：2-氧代丁酸至2-乙酰-2-羟丁酸的转化、2-乙酰-2-羟丁酸至2,3-二羟基-3-甲基戊酸的转化、和/或2,3-二羟基-3-甲基戊酸至α-酮基-3-甲基戊酸的转化。本发明也提供了生产反异脂肪酸的细胞以及使用所述细胞生产反异脂肪酸的方法。
摘要（英）：

A method for producing anteiso fatty acid is provided. The method comprises culturing a cell comprising at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes at least one of the following reactions: conversion of pyruvate to citramalate; conversion of citramalate to citraconate; conversion of citraconate to ss-methyl-D-malate; conversion of ss-methyl-D-malate to 2-oxobutanoate; or conversion of threonine to 2-oxobutanoate, under conditions allowing expression of the polynucleotide(s) and production of anteiso fatty acid. Optionally the cell further comprises at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes conversion of 2-oxobutanoate to 2-aceto-2-hydroxy-butyrate, conversion of 2-aceto-2-hydroxy-butyrate to 2,3-dihydroxy-3-methylvalerate, and/or conversion of 2,3-dihydroxy-3-methylvalerate to a-keto-3-methylvalerate. A cell that produces anteiso fatty acid and a method of using the cell to produce anteiso fatty acid also are provided.

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