The invention provides a double-layer skin covering material compositely built by collagen/fibrin glue-VEGF (vascular endothelial growth factor) and mesenchymal stem cell as well as a preparation method and the application of the double-layer skin covering material. Specifically, a double-layer tissue engineering support material is compositely built by collagen and VEGF-containing fibrin glue, and is hatched with the umbilical cord mesenchymal stem cells to prepare the biomimetic double-layer skin covering material. The double-layer skin covering material compositely built by the collagen/fibrin glue-VEGF (vascular endothelial growth factor) and the mesenchymal stem cell is proper in degradation property and skin growth rate, and controllable in mechanical property, thereby being a novel biomimetic skin covering material, so that the repair of the epidermis and the dermis can be promoted, the growth of the blood vessel endothelium can be promoted, the double-layer skin covering material is used for the repair of skin surface-layer and full-layer wounds, the union can be accelerated, an ideal wound repair effect can be achieved, and the double-layer skin covering material plays an important role in the repair of skin wound in clinic. Furthermore, the invention further discloses the application of the double-layer skin covering material.