The invention provides a color film substrate, a manufacturing method and a display device, which are used for solving the problems that the supporting capacity is reduced after the cross section area between a columnar spacer of the conventional color film substrate and one end connected with a black matrix is reduced. The color film substrate comprises a substrate, a black matrix and a columnar spacer, wherein the black matrix is formed on the substrate; the columnar spacer is formed above the black matrix; and the cross section area at two ends is smaller than that of a part between the two ends. In the technical scheme, the columnar spacer is hardly broken, the supporting capacity is high, and the thickness of a box can be guaranteed.