The invention relates to an embedding and spray drying method capable of remaining activity of beta-1, 3-1, 4-glucanase efficiently, which can realize long-term preservation of bi-1, 3-1, 4-glucanase at the room temperature while remaining activity thereof. The embedding and spray drying method mainly includes: filtering and concentrating crude enzyme liquid by an ultrafiltration membrane with the molecular weight of 10000 Daltons; adding adsorbing and embedding agent (beta-cyclodextrin), gelatin and sucrose into concentrated clear liquid, mixing the concentrated clear liquid by a magnetic mixer for at least 3 hours to lead the adsorbing and embedding agent to be mixed with the enzyme liquid uniformly to form suspension liquid; spraying the suspension by a centrifugal spray drying tower, and stirring the suspension continuously while feeding and drying so as to prevent the adsorbing and embedding agent from depositing. The operation conditions for spray drying include: the air inlet temperature is 180 DEG C, the air outlet temperature is 85 DEG C, and the embedded powder product of beta-1, 3-1, 4-glucanase can be obtained after the drying process.