Provided are a pneumatic tire configured in such a manner that air retention and film breakage are not caused even if a mold separation agent is not applied to the inner surface of the tire when the tire is vulcanized and molded using a bladder, and a method of manufacturing the pneumatic tire. The pneumatic tire is a pneumatic tire provided, on the inner surface thereof, with an inner liner (6) which uses a film layer. The pneumatic tire is manufactured by a manufacturing method including a step of pressing a non-vulcanized tire against molds (11, 13) by means of a bladder (14) without using a mold separation agent. The inner surface of the pneumatic tire consists of a film layer, and the dynamic friction coefficient between the film layer and the bladder (14) at a temperature of 25 DEG C is 2.0 or less.