The invention provides a tissue culture and rapid propagation method of Szechuan salvia miltiorrhiza bunge, wherein in the method, a tender stem section is utilized as an explant. The method comprises the following steps: selecting the explant; disinfecting the explant; carrying out induction culture; carrying out multiplication culture, carrying out rooting culture; and carrying out acclimatization and transplant. According to the tissue culture and rapid propagation method of Szechuan salvia miltiorrhiza bunge, a tissue culture technology is productively applied to the rapid propagation of Szechuan salvia miltiorrhiza bunge, and a culture method combining bud induction and bud mulitiplication is adopted, thus the browning of stem section tissues of Szechuan salvia miltiorrhiza bunge is effectively overcome and the browning is reduced by 20%. The tissue culture and rapid propagation method of Szechuan salvia miltiorrhiza bunge has the advantages of high bud induction rate and high transplant survival rate, wherein the bud induction rate can generally reach above 85% and the transplant survival rate can generally reach above 80%.