知搜-全球专利检索

  1. 登录
  2. 注册
发明公开
CN102212611A 黑麦草腥黑粉菌检测标准分子及其构建方法 失效 - 权利终止

黑麦草腥黑粉菌检测标准分子及其构建方法
摘要：
本发明公开了一种黑麦草腥黑粉菌检测的标准分子，为能够进行复制的质粒，且所述质粒中含有序列A(Seq ID No.1)和序列B(Seq ID No.2)中的至少一种。序列A与黑麦草腥黑粉菌线粒体DNA一段2.3kb的核酸序列同源性为99.65％，B与黑麦草腥黑粉菌核糖体内转录间隔区的DNA序列同源性为100％。本发明还公开了上述标准分子的制备方法和检测方法。本发明中构建的标准分子可以代替黑麦草腥黑粉菌DNA作为黑麦草腥黑粉菌分子检测的阳性对照，该标准分子适用于对黑麦草腥黑粉菌的定性PCR和定量PCR检测。该标准分子适合于口岸检验检疫、农业生产、植物保护等部门使用。
摘要（英）：

The invention discloses a standard molecule for detecting tilletia walkeri castebury&carris;. The standard molecule is a plasmid which can be duplicated, and the plasmid contains at least one of a sequence A (Seq ID N0.1) and a sequence B (Seq ID N0.2); the sequence A is 99.65% homologous with a 2.3kb nucleotide sequence of the mitochondrial DNA of the tilletia walkeri castebury&carris; and the sequence B is 100% homologous with the DNA sequence of a ribosomal internal transcribed spacer of the tilletia walkeri castebury&carris;. The invention also discloses a preparation method and detection method of the standard molecule. The standard molecule constructed in the invention can serve as a positive control for detecting the molecule of the tilletia walkeri castebury&carris; instead of the DNA of the tilletia walkeri castebury&carris; is suitable for qualitative and quantitative PCR (polymerase chain reaction) detection of the tilletia walkeri castebury&carris; and is suitable for the inspection and quarantine department of the port, the agricultural production department, the plant protection department and the like.

公开/授权文献
0/0