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发明公开
CN102210874A 一种HIV重组亚型DNA疫苗的制备方法 无效 - 撤回

一种HIV重组亚型DNA疫苗的制备方法
摘要：
本发明提供一种HIV重组亚型DNA疫苗的制备方法，通过以下步骤实现：设计与合成密码子优化后的融合基因序列，DNA疫苗的构建和体外表达能力测定。本发明以AE重组亚型HIV-1病毒为研究对象，构建包括env、pol、gag、tat、nef和rev基因在内的DNA疫苗，与AE-TRIVN相比，AE-Gp145TRN能够使特异性T细胞反应在Tat、Rev、Nef三个蛋白间更均匀的分布，提高了疫苗所活化的免疫反应广度。
摘要（英）：

The invention provides a preparation method of an HIV (Human Immunodeficiency Virus) recombinant subtype DNA (Deoxyribonucleic Acid) vaccine, realized by the following steps of: designing and synthesizing fusion gene sequences after codon optimization, constructing DNA vaccines and determining the expression ability in vitro. The preparation method takes AE recombinant subtype HIV-1 virus as a study object, and constructs the DNA vaccines including env, pol, gag, tat, nef and rev genes, and compared with AE-TRIVN, AE-Gp145TRN enables specific T cellular reaction to be distributed more evenly among Tat, Rev and Nef three proteins, thereby improving the immune reaction extent activated by the vaccine.

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