The invention provides an L-lysine fermentation method. The method comprises the following steps: introducing polynucleotides which code pyridine nucleotide transhydrogenase in a non-natural sequence into bacteria which can generate L-lysine to make the obtained bacteria to express the pyridine nucleotide transhydrogenase; and cultivating the obtained bacteria under a fermentation condition. The invention also provides intermediate products used in the fermentation method, and further product production with the fermentation, and the like.