The invention relates to a biological enzyme liquid/beverage multistage ultrafiltering-purifying-separating-concentrating device which comprises a raw material storage tank, a plate-type ultrafilter, a tubular ultrafilter, a filtrate gathering tank, a cam pump, a liquid pump, a flow regulator and a flowmeter, wherein a refrigeration coil of a refrigeration system is respectively arranged in the raw material storage tank and the filtrate gathering tank. By combining the plate-type ultrafiltering/tubular ultrafiltering multistage structures connected in parallel/series, arranging the multistagefiltering-concentrating devices and the close-type ultrafiltering system and matching with a perfect regeneration system, the ultrafiltering-separating device can operate at 5-35 DEG C so as to reduce the loss of enzyme activity and obtain a target product which is concentrated to the required concentration. In the process of filtering and concentrating, the plate-type ultrafiltering system or tubular ultrafiltering system is started according to the filtering level requirement to carry out single-stage or multistage filtering and concentrating, thereby obtaining the target product. The flow rate is regulated by frequency variation, so that the back flow circulating liquid and the permeation water can be used for implementing both separation of micromolecular substances and liquid concentrating, and continuous high-efficiency filtering can be implemented without choking the ultrafiltering tube, thereby enhancing the ultrafiltering treatment capacity, lowering the cost and increasing the yield.