The invention relates to a preparation method of an MCM-22 (Multiple Chip Module 22) molecular sieve, which comprises the following steps of: firstly preparing a structure directing agent, and uniformly mixing the structure directing agent with a silicon source, an aluminum source, a boron source or an iron source, an organic template agent and water into glue, wherein the organic template agent can be dimethyleneimine and can also be a mixed template agent comprising the dimethyleneimine and one or more of hydrocarbons, organic amine, alcohols and ketones; and then synthesizing an MCM-22 molecular sieve material through the silicon source, the aluminum source, alkali, the organic template agent and the structure directing agent, carrying out hydrothermal crystallization and obtaining an MCM-22 molecular sieve product through conventional processes of filtering, washing, drying and roasting. The method disclosed by the invention has the main characteristic that the structure directingagent is added into gel, and compared with the prior art, the method disclosed by the invention has the advantages of low crystallization temperature, short crystallization time, high product yield, high product quality and the like. The MCM-22 molecular sieve product disclosed by the invention has excellent catalytic performances, such as catalytic cracking, olefin and benzene alkylation and thelike.