知搜-全球专利检索

  1. 登录
  2. 注册
发明授权
CN101988128B 一种用于检测CYP2C19基因分型的PCR试剂盒 有权 转让

一种用于检测CYP2C19基因分型的PCR试剂盒
摘要：
一种用于CYP2C19基因分型的PCR试剂盒，采用一系列经修饰的检测引物以及高保真DNA聚合酶介导的聚合酶链式反应，检测基因CYP2C19*1/*2型（即681G/A位点）和/或CYP2C19*1/*3型（即636G/A位点）。本发明的试剂盒，可以实现低成本、高效率、高通量检测CYP2C19基因中影响其编码的药物代谢酶活性的两个位点的碱基类型。这两个位点的突变可以解释中国人群相关药物的弱代谢者，本发明的试剂盒的检测结果可以用于指导个体化用药。
摘要（英）：

The invention discloses a PCR kit for detecting CYP2C19 genotyping. In the invention, a series of modified detection primers and high-fidelity DNA polymerase mediated PCR are adopted to detect the gene CYP2C19*1/*2 type (namely, the 681 G/A locus) and/or the CYP2C19*1/*3 type (namely, the 636 G/A locus). The kit of the invention can realize that the base types of the two locuses influencing the activity of drug metablic enzyme coded by the CYP2C19 gene in the CYP2C19 gene with low cost, high efficiency and high flux. The mutation of the two locuses can explain the weak metabolism of Chinese people for associated drugs, and the detection result of the kit of the invention can be used for instructing the individual drug.

公开/授权文献
0/0