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CN101979503A 表达AsiaI型口蹄疫病毒VP1基因的重组布鲁氏菌及其疫苗的生产方法 失效 - 权利终止

表达AsiaI型口蹄疫病毒VP1基因的重组布鲁氏菌及其疫苗的生产方法
摘要：
本发明涉及表达Asia I型口蹄疫病毒VP1基因的重组布鲁氏菌及其疫苗的生产方法。该重组菌株通过在布鲁氏菌S2株菌基因组中稳定地整合进Asia I口蹄疫病毒(FMDV)江苏株VP1基因，并同时破坏了光滑型布鲁氏菌细胞壁LPS结构中O链形成的条件，使重组菌由光滑型转变为粗糙型，使菌株的安全性进一步提高，但仍保留了对布鲁氏菌病良好的免疫效果，该重组菌被命名为重组布鲁氏菌rS2-JS株。该菌能够表达Asia I FMDV VP1蛋白，并诱导相应抗体产生，对Asia I FMD有良好的基础免疫作用。以此菌株生产疫苗将改变布鲁氏菌疫苗免疫动物与野毒株感染动物难以区分的现状，同时实现了FMD疫苗的细胞免疫，并将为布鲁氏菌病和FMD的防控提供了一种良好的疫苗。
摘要（英）：

The invention relates to recombinant brucella for expressing Asia type-I foot and mouth disease virus VP1 genes and a method for producing vaccines thereof. Asia type-I foot and mouth disease virus (FMDV) Jiangsu strain VP1 genes are integrated into a brucella S2 strain genome, and the forming condition of an O chain in a smooth brucella cell wall LPS structure is simultaneously damaged so that the recombinant strain is changed into a rough type from a smooth type, the safety of the strain is further improved, but good immune effect on the brucella is still kept; and the recombinant strain is named as a recombinant brucella rS2-JS strain. The strain can express the Asia I FMDV VP1 protein, induces the generation of corresponding antibodies and has good basic immune effect on the Asia I FMD. The strain for producing the vaccines can change the current situation that brucella vaccine immunized animals and wild strain infected animals are difficult to distinguish, realizes cellular immunity of FMD vaccines at the same time, and provides a good vaccine for prevention and control of brucella diseases and FMD.

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