The invention discloses a method for preparing anti-taxol monoclonal antibody, relates to the method for preparing the monoclonal antibody, and solves the problems that: the taxol yield of the strain in the conventional taxol microbial fermentation is still low, the impurity content of the fermentation liquor is high and the taxol is difficult to separate and extract. The method comprises the following steps of: synthesizing and identifying taxol artificial antigen; 2, immunizing Balb/c mice by using synthesized taxol immunogen, fusing the mice spleen cells with high titer and high specificity with SP2/0 cells, and culturing and screening cell strains; and 3, injecting the cell strains into the abdominal cavity of the mice and harvesting the ascitic fluid so as to obtain the high-concentration monoclonal antibody. The yield of the anti-taxol monoclonal antibody can reach 1 to 12,800, the impurity content of the fermentation liquor is low, the taxol is simple to separate and extract, and a foundation is laid for quickly and efficiently separating, extracting and purifying the taxol from endophytic fungi fermentation liquor, and realizing industrial production at an early date.