知搜-全球专利检索

  1. 登录
  2. 注册
发明授权
CN101942471B 一种环氧化物水解酶及其制备方法 有权

一种环氧化物水解酶及其制备方法
摘要：
本发明公开了一种环氧化物水解酶及其制备方法。该环氧化物水解酶的氨基酸序列的第163位的氨基酸为苯丙氨酸，其氨基酸序列如SEQ ID NO:10所示，该环氧化物水解酶由核苷酸序列如SEQ ID NO:9所示的DNA分子所编码。该环氧化物水解酶的制备方法是用苯丙氨酸取代野生型环氧化物水解酶的氨基酸序列第163位的酪氨酸，所述野生型环氧化物水解酶的氨基酸序列如SEQ ID NO:4所示，该野生型环氧化物水解酶由核苷酸序列如SEQ ID NO:3所示的DNA分子所编码。与野生型环氧化物水解酶相比，本发明环氧化物水解酶具有较高的温度稳定性并维持高酶活性，可用于水解顺式环氧琥珀酸或其盐为L(+)-酒石酸或其盐，提高工业化生产中生物催化剂的使用寿命。
摘要（英）：

The invention discloses an epoxide hydrolase and a preparation method thereof. The amino acid of the 163rd site of the amino acid sequence of the epoxide hydrolase is phenylalanine with the amino acid sequence expressed by SEQ ID No: 10, and the epoxide hydrolase is coded by DNA molecules (expressed as SEQ ID No: 9) of the amino acid sequence. The preparation method for the epoxide hydrolase comprises that: tyrosine of the 163rd site of the amino acid sequence of the wild epoxide hydrolase is substituted by using the phenylalanine, the amino acid sequence of the wild epoxide hydrolase is SEQ ID No: 4, and the wild epoxide hydrolase is coded by DNA molecules (expressed as SEQ ID No: 3) of the amino acid sequence. Compared with the wild epoxide hydrolase, the epoxide hydrolase has high temperature stability and maintains high enzyme activity, can be used for hydrolyzing cis-epoxysuccinic acid or L (+)-tartaric acid or salt thereof, and prolongs the service life of a biocatalyst in the industrialized production.

公开/授权文献
0/0