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发明公开
CN101914532A RNA干扰的目标碱基序列的搜索方法 无效 - 撤回

RNA干扰的目标碱基序列的搜索方法
摘要：
本发明涉及RNA干扰的目标碱基序列的搜索方法。本发明从RNA干扰的目标基因的碱基序列中搜索符合下述规则(a)-(d)的序列部位，根据搜索结果进行能够产生RNA干扰的小干扰RNA(siRNA)的设计、合成等。(a)3’末端的碱基是腺嘌呤(A)、胸腺嘧啶(T)或尿嘧啶(U)。(b)5’末端的碱基是鸟嘌呤(G)或胞嘧啶(C)。(c)3’末端的7碱基序列中富含由腺嘌呤(A)、胸腺嘧啶(T)和尿嘧啶(U)组成的群组中选择的1或1种以上的碱基。(d)碱基数在产生RNA干扰且不产生细胞毒性的范围内。
摘要（英）：

In the present invention, a sequence segment conforming to the following rules (a) to (d) is searched from the base sequences of a target gene of RNA interference and, based on the search results, siRNA capable of causing RNAi is designed, synthesized, etc.: (a) The 3' end base is adenine, thymine, or uracil, (b) The 5' end base is guanine or cytosine, (c) A 7-base sequence from the 3' end is rich in one or more types of bases selected from the group consisting of adenine, thymine, and uracil, and (d) The number of bases is within a range that allows RNA interference to occur without causing cytotoxicity.

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