The invention discloses a protein chip for detecting transgenic products. The protein chip is a film which is arranged on the plane of a plane type supporting carrier and coated with a modified material; and protein antibody probes for detecting transgenic agricultural products are arranged on the surface of the film in a lattice mode and comprise 1) a Bt cry1Ac/Ab monoclonal antibody, 2) a Bt cry1Ah monoclonal antibody, 3) a phytase PHY monoclonal antibody, 4) a glyphosate-tolerant G2-EPSPS polyclonal antibody and 5) a salt-tolerant IrrE polyclonal antibody. The invention further provides a chip kit for detecting transgenic crops and performing result judgment simply and rapidly based on antigen antibody reaction, which belongs to the field of inspection and quarantine. The protein chip kit has the advantages of: 1) high speed, wherein the detection time is only 1.5 to 2 hours; 2) specificity, wherein protein antigens and antibodies are specifically combined and each antibody performs a point system process repeatedly for three times, so that the result correctness is greatly improved; 3) high flux, wherein the protein chip kit can detect the proteins of five kinds of commercial transgenic components at the same time; and 4) stability, wherein the results of the repeated tests show that the stability of the method is high.