The invention provides array optical tweezers based on multicore polarization-preserving fiber and a manufacturing method thereof. The array optical tweezers comprise the multicore polarization-preserving fiber, standard single-mode fiber and a laser light source, wherein the laser light source is connected with one end of the standard single-mode fiber, the other end of the standard single-mode fiber is connected with the multicore polarization-preserving fiber in a fusion conical pull coupling mode, and the other end of the multicore polarization-preserving fiber is manufactured into a centrum shape through fusion conical pull processing equipment. In the invention, a plurality of optical waveguide fiber cores are integrated in one fiber, thus not only saving physical space but also significantly reducing system input light power and reducing harm on particles to be captured. Meanwhile, the multicore fiber composite optical tweezers can capture particles more flexibly and accurately, and has adjustability, thus greatly improving the practicability of fiber and optical tweezers technologies. More importantly, the array optical tweezers can form a compact interference grid opticalfield array, so as to form optical potential wells at coherence enforcement points to realize functions such as filtering particles.