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发明公开
CN101892236A 靶向ZNF268基因的RNA干扰表达载体构建及应用 失效 - 权利终止

靶向ZNF268基因的RNA干扰表达载体构建及应用
摘要：
本发明属于分子生物学领域，公开了靶向ZNF268基因的RNA干扰表达载体构建方法及其应用，本发明方法是将筛选得到的靶向ZNF268基因的siRNA寡核苷酸片段插入到载体系统中，构建成靶向ZNF268基因的RNA干扰质粒载体。实验表明，本发明构建的表达载体可以在肿瘤细胞中高效表达特异的siRNA序列，且该序列能够结合ZNF268基因的mRNA，使ZNF268基因的表达量显著降低，达到抑制ZNF268基因表达的目的。
摘要（英）：

The invention belongs to the field of molecular biology and discloses a construction method of an RNA interference expression vector of a targeted ZNF268 gene and application thereof. In the method, screened siRNA oligonucleotide segments of the targeted ZNF268 gene are inserted into a vector system to construct an RNA interference plasmid vector of the targeted ZNF268 gene. Proved by experiments, the constructed expression vector can efficiently express a specific siRNA sequence in a tumor cell, and the sequence can be combined with an amRNA of the ZNF268 gene to ensure that the expression quantity of the ZNF269 gene is obviously reduced so as to achieve the purpose of suppressing the expression of the ZNF268 gene.

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