The invention discloses a tomato glutaredoxin gene SlRX1 as well as a cloning method and application thereof. The tomato glutaredoxin gene SlRX1 is a first glutaredoxin gene separated from tomatoes, consists of 1,003 basic groups and is used for coding CGFS glutaredoxin forced and induced in environments of drought, high salt, and the like. The gene can be expressed in roots, stems, leaves and petals of the tomatoes and participates in an anti-nonbiostress reaction of plants. If the gene is silent in tomatoes, the tomatoes are more sensitive to the environments draught, high salt, oxidativestress, and the like, and if the gene is excessively expressed in arabidopsis thaliana, the resistance to the environments of draught, high salt and oxidativestress of transgenic arabidopsis thaliana. The gene can improve the resistance of plants to the environments of draught, high salt and oxidativestress and has strong theoretical research and application values.