知搜-全球专利检索

  1. 登录
  2. 注册
发明公开
CN101838618A 一种高α-半乳糖苷类寡糖降解能力的中性α-半乳糖苷酶Aga-S27及其基因和应用 无效 - 撤回

一种高α-半乳糖苷类寡糖降解能力的中性α-半乳糖苷酶Aga-S27及其基因和应用
摘要：
本发明涉及基因工程领域，具体地，本发明提供了一种高α-半乳糖苷类寡糖降解能力的中性α-半乳糖苷酶Aga-S27及其基因和应用。本发明筛选得到新的链霉菌Streptomyces sp.S27，其保藏号为CGMCC No.3146，并从该菌株中得到一种对α-半乳糖苷类寡糖具有高降解能力的中性α-半乳糖苷酶Aga-S27，进而得到其基因、包含该基因的重组载体。所述α-半乳糖苷酶Aga-S27的氨基酸序列如SEQ ID NO.1所示，该酶具有适宜的作用温度及pH值，较强的蛋白酶抗性和较好的水解各种底物的能力，可作为一种饲料或食品添加剂应用于饲料与食品行业。
摘要（英）：

The invention relates to the field of genetic engineering and particularly provides neutral alpha-galactosidase Aga-S27 having high degradability of alpha-galactoside oligosaccharide, and genes and application thereof. The neutral alpha-galactosidase Aga-S27 having high degradability of alpha-galactoside oligosaccharide can be obtained from a bacterial strain, i.e., novel streptomyces sp.S27 (CGMCC No.3146) screened by the invention, and the genes thereof and recombinant vector containing the genes can be further obtained, wherein the amino acid sequences of alpha-galactosidase Aga-S27 are shown in SEQ ID No.1. The alpha-galactosidase Aga-S27 has the advantages of appropriate operating temperature and pH value, higher protease resistance and higher ability to hydrolyze various substrates. Therefore, the alpha-galactosidase Aga-S27 is applicable as feed or food additives in the industries of feed and food.

0/0