The invention relates to a detection primer of phakospora sojae sawada, a kit and a real-time PCR (polymerase chain reaction) detection method. The primer comprises a forward primer Rs-F and a reverse primer Rs-R, wherein the sequence of the forward primer Rs-F is 5'-GTGCACTTTATTGTGGCTCAAAACT-3', and the sequence of the reverse primer is 5'-ATGATTAATAGGTGGGTTGCAGC-3'. The detection method sequentially comprises the following steps of: synthesizing the primer, adding the primer to an object to be detected and carrying out an amplification reaction, wherein the amplification reaction comprises the following steps of: carrying out pre-denaturation; repeatedly carrying out a reaction interval; and carrying out HRM (High Resolution Melt) analysis on a reaction result automatically recorded by a PCR instrument. The invention has the advantages of simple, quick and accurate detection, strong specificity, high sensitivity, good repeatability and easy popularization and application, can be widely applied to carryING out the bioassay or the detection of phakospora sojae sawada molecules found in the processes of actual quarantines and field surveys and is particularly suitable for occasionshaving particularly high requirement on timeliness, such as port quarantines, and the like.