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发明公开
CN101768640A 河豚鱼成分荧光定量PCR检测试剂及其制备方法和应用 有权

河豚鱼成分荧光定量PCR检测试剂及其制备方法和应用
摘要：
本发明公开了河豚鱼成分荧光定量PCR检测试剂及其制备方法和应用，系选择不同种河豚鱼之间比较保守的细胞色素B基因片段为靶目标序列，经过精心设计并合成，包含一对特异性引物和一条特异性荧光探针，扩增目标片段长度为102bp，引物和探针序列为：引物：Takifugu？TF：5′-TCTTCACGAAACAGGCTCCAAC-3′，Takifugu？TR：5′-GTGAAGCCCAGGAGGTCTTTGTA-3′，探针：5′-FAM-CGCAGACAAAATCCCATTCCACCCATA-TAMRA-3′；本发明是一种对混入鱼糜制品中的河豚鱼成分具有特异性高、敏感性强，快速检测的荧光定量PCR检测试剂；本发明的方法设计合理，定性准确。
摘要（英）：

The invention discloses a puffer fish ingredient fluorescence quantitative PCR detection reagent and a preparation method and application thereof, in the technical scheme, relatively conservative cytochrome B gene fragments of different puffer fishes are selected to be the target sequences, after elaborately design and synthesis, a pair of specific primer and a specific fluorescent probe are contained, the length of the target segment is enlarged to 102bp, the sequences of the primer and the probe are respectively as follows: primer: Takifugu TF:5'-TCTTCACGAAACAGGCTCCAAC-3', Takifugu TR: 5'-GTGAAGCCCAGGAGGTCTTTGTA-3'; and probe: 5'-FAM-CGCAGACAAAATCCCATTCCACCCATA-TAMRA-3'. The invention is the fluorescence quantitative PCR detection reagent with high specificity, strong sensibility and rapid detection to the puffer fish ingredient mixed with minced fish products; and the method of the invention has reasonable design and accurate definiteness.

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