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发明公开
CN101768600A RegIII/胰岛素原双基因质粒及其构建方法和应用 无效 - 撤回

RegIII/胰岛素原双基因质粒及其构建方法和应用
摘要：
本发明提供了一种用于1型糖尿病治疗的质粒，该质粒是通过基因重组方法构建的含有胰岛β细胞再生(Reg)III基因和胰岛素原基因的双基因真核共表达质粒，其中RegIII基因和胰岛素原基因可分别在pBudCE4.1质粒载体中EF-1α和CMV启动子的引导下进行表达。RegIII/胰岛素原双基因真核共表达质粒可通过恢复1型糖尿病患者体内自身免疫耐受状态和促进β细胞的再生而发挥降糖作用。
摘要（英）：

The invention provides a plasmid for treating 1 type diabetes, which is a double-gene eukaryon co-expression plasmid built by a gene recombination method and containing a pancreas islet beta cell regeneration (Reg) III gene and a proinsulin gene, wherein the Reg III gene and the proinsulin gene can be respectively expressed under the induction of an EF-1alpha promoter and a cytomegalovirus (CMV) promoter of a pBudCE4.1 plasmid vector. The Reg III/proinsulin double-gene eukaryon co-expression plasmid can play a hypoglycemic role by recovering the autoimmunity tolerance state of in the body of a 1 type diabetes patient and promoting the regeneration of beta cells.

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