The invention discloses a quick test kit of schistosomiasis and a preparation method thereof. A water absorbing pad, a pyroxylin film, an aerosol bonding pad and a sample pad are and sequentially connected in horizontal direction and fixed on a baseplate which is arranged in a kit body, and the kit body is provided with an observation window corresponding to the pyroxylin film and a sample loading hole corresponding to the sample pad. Only an one-step operation is needed when the quick test kit is used, and the quick test kit not only can detect serum and plasma samples, but also can detect awhole blood sample, thereby avoiding the step of obtaining the serum sample which can be obtained only through a centrifugation step from a blood sample, and greatly improving the screening speed. The quick test kit can also detect whether animals such as cows, horses, pigs, rabbits, and the like have the schistosomiasis or not so as to overcome the defect that different kits are needed to be prepared for detecting different species of other kits.